Lineweaver burke plot Study guides, Class notes & Summaries

First Aid USMLE Step 1: Pharmacology 2023 In the Michaelis-Menten kinetics what do the following mean: Km Vmax Draw a typical hyperbolic curve on a Michaelis-Menten Plot, then draw how the addition of a competitive and non-competitive inhibitor would change the kinetics of the reaction. - ANS-*In the Michaelis-Menten kinetics what do the following mean:* Km: - the concentration of substrate at 1/2 Vmax - this is INVERSELY related to the affinity of enzyme for its substrate Vmax: ...

Biochemistry NBME Exam 2024 Well Revised and Verified Answers Updated Proteoglycans Act as molecular sieves in ECM, CT, and cartilage GAGs are the long polysacc chains attached to core protein GAG is heavily hydrated and cushions (has "-" charge) Degraded via lysosomal pathway (defect = MPS) Glycolipids A.K.A. sphingolipids Made with ceramide lipid base Serine + palmitoyl-CoA => sphingosine (+FFA) => ceramide Carb part sticks out into ECM (same w/ glycoproteins) Side chain p...

In the Michaelis-Menten kinetics what do the following mean: Km Vmax Draw a typical hyperbolic curve on a Michaelis-Menten Plot, then draw how the addition of a competitive and non-competitive inhibitor would change the kinetics of the reaction.*In the Michaelis-Menten kinetics what do the following mean:* Km: - the concentration of substrate at 1/2 Vmax - this is INVERSELY related to the affinity of enzyme for its substrate Vmax: - maximal velocity at which enzymatic reaction occurs ...

In the Michaelis-Menten kinetics what do the following mean: Km Vmax Draw a typical hyperbolic curve on a Michaelis-Menten Plot, then draw how the addition of a competitive and non-competitive inhibitor would change the kinetics of the reaction.*In the Michaelis-Menten kinetics what do the following mean:* Km: - the concentration of substrate at 1/2 Vmax - this is INVERSELY related to the affinity of enzyme for its substrate Vmax: - maximal velocity at which enzymatic reaction occurs ...

In the Michaelis-Menten kinetics what do the following mean: Km Vmax Draw a typical hyperbolic curve on a Michaelis-Menten Plot, then draw how the addition of a competitive and non-competitive inhibitor would change the kinetics of the reaction.*In the Michaelis-Menten kinetics what do the following mean:* Km: - the concentration of substrate at 1/2 Vmax - this is INVERSELY related to the affinity of enzyme for its substrate Vmax: - maximal velocity at which enzymatic reaction occurs ...

In the Michaelis-Menten kinetics what do the following mean: Km Vmax Draw a typical hyperbolic curve on a Michaelis-Menten Plot, then draw how the addition of a competitive and non-competitive inhibitor would change the kinetics of the reaction.*In the Michaelis-Menten kinetics what do the following mean:* Km: - the concentration of substrate at 1/2 Vmax - this is INVERSELY related to the affinity of enzyme for its substrate Vmax: - maximal velocity at which enzymatic reaction occurs ...

In the Michaelis-Menten kinetics what do the following mean: Km Vmax Draw a typical hyperbolic curve on a Michaelis-Menten Plot, then draw how the addition of a competitive and non-competitive inhibitor would change the kinetics of the reaction.*In the Michaelis-Menten kinetics what do the following mean:* Km: - the concentration of substrate at 1/2 Vmax - this is INVERSELY related to the affinity of enzyme for its substrate Vmax: - maximal velocity at which enzymatic reaction occu...

In the Michaelis-Menten kinetics what do the following mean: Km Vmax Draw a typical hyperbolic curve on a Michaelis-Menten Plot, then draw how the addition of a competitive and non-competitive inhibitor would change the kinetics of the reaction.*In the Michaelis-Menten kinetics what do the following mean:* Km: - the concentration of substrate at 1/2 Vmax - this is INVERSELY related to the affinity of enzyme for its substrate Vmax: - maximal velocity at which enzymatic reaction occu...

ACS Biochemistry Comps EXAM QUESTIONS WITH COMPLETE SOLUTIONS Ion-Exchange Chromatography - ANSWERS Separates sample based on charge. CM attracts +, DEAE attracts -. May have repulsion effect on like charges. Salt or acid used to remove stuck proteins. Hydrophobic/Reverse Phase Chromatography - ANSWERS Beads are coated with a carbon chain. Hydrophobic proteins stick better. Elute with non-H-bonding solvent (acetonitrile). Affinity Chromatography - ANSWERS A...

This summary covers all the details about enzyme kinetics and essential equations. It covers the Mechaelis-Menteen, Km, catalytic efficiency, how to analyse kinetic data/curves, Lineweaver-Burke plot, Hofstee-Eadie plot, Hanes-Woolf plot, and calculation from kinetic data.